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This study was conducted to establish the induction and proliferation of callus in Lilium Oriental Hybrids. Callus induction from microscales of Lilium Oriental Hybrid ´Casa Blanca´ leaded 80 to 100% on MS solid medium supplemented with 0.25 to 5.0 μM 2,4-D and 0.05 to 0.5 μM BA. Callus formation from floral organs was 33.5% in ´Acapulco´, 14.5% in ´Casa Blanca´ and ´Star Gazer´, and 12.5% in ´Le Reve´. Callus formation from different floral organs was from 3.0 to 77.0%, and was best in filament followed by peduncle, style, ovary, inner tepal, outer tepal and anther. Calli were generally produced on cut surfaces of microscale and floral organ after 2-4 months in culture, and in some case callus could be developed from those tissues after once explants were brownish. Callus induced under the dark condition was light yellow, and under the light condition was light green in color. Light and dark conditions had no effects on induction and proliferation of callus, and sucrose concentrations such as 30 g·L^(-1) and 60 g·L^(-1) were favorable to callus proliferation. The requirement of exogenous growth regulators was reduced with increase in callus subculture. It was possible to maintain and multiply callus until the tenth subculture, regardless of light or dark condition.
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